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You are here: Home > Community > Scientific Services > Water Testing > Microbiological examination of drinking waters
Escherichia coli Bacteria

Microbiological examination of drinking waters

It is very important to ensure that drinking water is not contaminated with micro-organisms. We determine whether there is possible contamination of water with agents that might cause illness or disease by checking whether there are increased levels of faecal coliforms.
We measure the total colony count and levels of total and faecal coliforms present in water for comparison against the appropriate Regulations.
A summary of the recommended levels can be downloaded as a pdf (46 Kb).
Please ask us if you would like further explanation of your test results.

Regulations

Various Regulations control the microbiological safety of water for human consumption ie, The Natural Mineral Water, Springwater and Bottled Drinking Water Regulations 1999, The Water Supply (Water Quality) Regulations 2000 and The Private Water Supplies Regulations 1991.
Here are the Microbiological Guidelines and Standards for Drinking Water
Type of Water
Parameters
Permitted Concentration or Value
Guide Level
Private Supplies      
Classes A to E
and 1 to 4
coliforms/E. coli
colony counts at 22ºC and 37ºC
0/100 mL No significant increase over normal levels.
See note (a)
No organism detrimental to public health
Class F coliforms/E. coli 0/100 mL No significant increase over normal levels.
See note (a)
No organism detrimental to public health
Natural Mineral Waters      
sampled any time up to sale coliforms/E. coli
enterococci
Pseudomonas aeruginosa
sulphite reducing clostridia
parasites/pathogens
0/250 mL
0/250 mL
0/250 mL
0/50 mL
Absent
 
within 12 h of bottling As above plus
colony count at 22ºC/72 h
colony count at 37ºC/48 h
.
100/mL
20/mL
 
Drinking Water in Containers      
at any time coliforms/E. coli
enterococci
Pseudomonas aeruginosa
sulphite reducing clostridia
colony counts at 22ºC and 37ºC
0/100 mL
0/100 mL
0/100 mL
0/20 mL
Should show no appreciable increase after bottling
 
within 12 h of bottling As above plus
colony count at 22ºC/72 h
colony count at 37ºC/48 h
.
100/mL
20/mL
.
20/mL
5/mL
Vending Machines coliforms/E. coli <10/mL  
(a) For colony counts on drinking waters some of our customers use guideline levels of <10 colonies/mL at 37ºC and <100 colonies/mL at 22ºC.

Additional Tests

The Leicestershire Scientific Services also provides a comprehensive chemical testing service on waters. Please contact us for more details.
scientificservices@leics.gov.uk       0116 305 5640

further information

Contact : Scientific Services
Telephone : 0116 305 5640
Fax : 0116 305 5660
E-Mail : scientificservices@leics.gov.uk
Last Updated:
15 April 2008
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